This kit simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-based purification. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the EZ-10 column with a silica membrane. RNA binds to the silica membrane, all protein and other components are removed in the flow-through. Remaining contaminants and salts are efficiently washed away.
Purified RNA has OD260/OD280 ratios of 1.9-2.1
Purified RNA is ideal for use in most downstream applications including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.
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