This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products. Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for intercalator-based qPCR. Additionally, SYBR Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.
Takara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. SYBR Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.
High specificity minimizes primer-dimer formation and nonspecific amplification
Excels with GC-rich targets
2X master mix provides convenience
High sensitivity: detects fewer than 100 copies
Compatible with all commonly used qPCR instruments
Includes SYBR Green Master Mix for intercalator-based qPCR
Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
Eliminates need for separate RNase H digestion step when using low RNase H RTs
Gene expression analysis
Suppression of primer-dimers or off-target products
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