The total Protein extraction Kit provides an optimized cell lysis buffer, protease inhibitor cocktail, and Phosphatase inhibitor for convenient extraction of mammalian proteins from cultured cells and tissue samples, under non-denaturing conditions. Total protein isolated by this kit maintain biological activity and can be used for many downstream applications including SDS-PAGE, Western Blotting, IP, Pull Down, Gel Mobility Shift, protein assays and so on. The kit is sufficient to extract proteins
from 50x mammalian cells or 50x200mg of tissues sample.
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