Tsg has a half life of 3 hours at 95oC, it is therefore more stable than Tsg DNA Polymerase. Tsg has high fidelity with an error frequency one in a million base pairs during DNA synthesis. Tsg is designed for use in primer extension reaction. Tsg can also be used for sequencing.
Tsg has the independent terminal transferase activity which results in the addition of a single nucleotide (adenosine) at 3' end of the extension product. TA cloning vector is recommended if the extension product is needed to be cloned.
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