EZ-10 Column Blood RNA Purification Kit provides a simple spin column technique for preparation of high quality, high-purity intact total RNA. The reagent contains disruptive and protective properties of guanidine isothiocyanate and β-mercaptoethanol to inactivate the ribonucleases present in cell extracts. RNA in the whole homogeneity is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-free water.
5-15 μg total RNA can be purified from 200 μl of anticoagulated blood sample using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) selection and in vitro translation.
Fast. Using a rapid spin-column format, the entire procedure takes approx 15 minutes.
High Purity of RNA. OD260/OD280 ratio of purified RNA is generally > 1.9.
Compatible with downstream applications such as Northern Blots, cDNA synthesis, RT-PCR and qRT-PCR.
High Quality RNA. Buffer Rlysis-AG maintains the integrity the RNA, no degradation.
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