HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture.
SOLUBILITY / SOLUTION STABILITY:
A 1 M solution (954 mg in 4 mL water) 1639 mOsm/kg is clear and colorless, with pH approximately between 5.0 and 6.5 at 20°C.1 At 0°C, a saturated solution is reportedly 2.25 M. Solutions may be autoclaved under standard conditions.
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